SRR1299755

SRA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

77/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read RNA-seq (HiSeq 2500), grade C—marginal for reuse. High adapter content (22.54%) and moderate Q30 (88.2%) reflect older sequencing-era quality challenges; should undergo rigorous adapter removal and quality filtering before downstream use.

Data type / assay

bulk-RNA-seq

Organism

Anigraea

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 4009877400 reported

total reads 13366258 reported

n content pct 0 measured

pct q20 bases 94.8 measured

pct q30 bases 88.2 measured

gc content pct 41.8 measured

mean read length 150 measured

mean base quality 34.3 measured

adapter content pct 22.54 measured

duplication rate pct 36.87 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 77/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.2 measured ×1 91%

mean base quality 34.3 measured ×0.6 100%

adapter content pct 22.54 measured ×0.4 0%

duplication rate pct 36.87 measured ×0.4 85%

QC cost 41 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0