SRR13082984

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

miRNA-seq (Bemisia tabaci), Grade D. Adapter at 98.84% and duplication at 75.94% (both scored 0) indicate library failure. On 50 bp reads, 98.84% adapter means genuine inserts are essentially absent, making miRNA expression quantification impossible.

Data type / assay

bulk-RNA-seq

Organism

Bemisia tabaci

Instrument

Illumina NovaSeq 6000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1309113700 reported

total reads 26182274 reported

n content pct 0.004 measured

pct q20 bases 99.3 measured

pct q30 bases 97.5 measured

gc content pct 49.3 measured

mean read length 50 measured

mean base quality 36.6 measured

adapter content pct 98.84 measured

duplication rate pct 75.94 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.5 measured ×1 100%

mean base quality 36.6 measured ×0.6 100%

adapter content pct 98.84 measured ×0.4 0%

duplication rate pct 75.94 measured ×0.4 0%

QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0