SRR1313735

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

89/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from H. sapiens, grade B. Measured duplication at 55.76% (43/100) is severely elevated and will substantially inflate transcript abundance if not addressed. Q30 (89.1%) is acceptable but lower than ideal, compounding reliability concerns for high-confidence expression quantification.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 903806300 reported

total reads 9038063 reported

n content pct 0.006 measured

pct q20 bases 94.1 measured

pct q30 bases 89.1 measured

gc content pct 56.6 measured

mean read length 100 measured

mean base quality 34.6 measured

adapter content pct 0.07 measured

duplication rate pct 55.76 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 89/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 89.1 measured ×1 96%

mean base quality 34.6 measured ×0.6 100%

adapter content pct 0.07 measured ×0.4 100%

duplication rate pct 55.76 measured ×0.4 43%

QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0