SRR1313736

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

84/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from H. sapiens, grade B. Measured duplication at 57.82% (38/100) is very high and will substantially inflate expression estimates if not addressed; Q30 (87.4%) is also lower than ideal, compounding reliability concerns.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 2618865600 reported

total reads 26188656 reported

n content pct 0.015 measured

pct q20 bases 92.7 measured

pct q30 bases 87.4 measured

gc content pct 57.4 measured

mean read length 100 measured

mean base quality 34.1 measured

adapter content pct 0.02 measured

duplication rate pct 57.82 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 84/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 87.4 measured ×1 87%

mean base quality 34.1 measured ×0.6 100%

adapter content pct 0.02 measured ×0.4 100%

duplication rate pct 57.82 measured ×0.4 38%

QC cost 22 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0