Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR13149619

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

88/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq from insect (Riptortus pedestris) on NovaSeq 6000. Grade B (88/100) with excellent base composition (Q20 98.3%, Q30 94.8%, adapters 0%) but duplication at 63.7% is critical, scoring only 25/100 and dominating the grade reduction. All metrics measured; the high duplication suggests potential PCR or library bias that could skew expression estimates and limit reusability for quantitative studies.

Data type / assay
bulk-RNA-seq
Organism
Riptortus pedestris
Instrument
Illumina NovaSeq 6000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 9693745500 reported
total reads 32312485 reported
n content pct 0 measured
pct q20 bases 98.3 measured
pct q30 bases 94.8 measured
gc content pct 43 measured
mean read length 150 measured
mean base quality 36.1 measured
adapter content pct 0 measured
duplication rate pct 63.7 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 88/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.8 measured ×1 100%
mean base quality 36.1 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 63.7 measured ×0.4 25%
QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0