Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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SRR13258422

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

48/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

NextSeq 550 short-read RNA-seq of Hormaphis cornu (aphid) yielding 10.3 million reads at lower quality (Q30=75.4%) across 3.1 billion bases. The dataset can support gene expression studies with appropriate quality control, though the relatively low base quality suggests greater care needed in read mapping and variant calling.

Data type / assay
bulk-RNA-seq
Organism
Hormaphis cornu
Instrument
NextSeq 550
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 3079310700 reported
total reads 10264369 reported
n content pct 0.001 measured
pct q20 bases 81 measured
pct q30 bases 75.4 measured
gc content pct 44.2 measured
mean read length 150 measured
mean base quality 30.6 measured
adapter content pct 5.83 measured
duplication rate pct 40.44 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 48/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 75.4 measured ×1 27%
mean base quality 30.6 measured ×0.6 43%
adapter content pct 5.83 measured ×0.4 79%
duplication rate pct 40.44 measured ×0.4 77%
QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0