SRR13258426

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

56/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

NextSeq 550 bulk RNA-Seq of Hormaphis cornu generated 24.2M reads with moderate quality (78.5% Q30) and elevated GC content (52.1%), suggesting potential systematic bias. Despite quality limitations, this dataset provides gene expression profiling for symbiont-containing aphid tissues with sufficient depth for major transcript detection.

Data type / assay

bulk-RNA-seq

Organism

Hormaphis cornu

Instrument

NextSeq 550

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 7264497900 reported

total reads 24214993 reported

n content pct 0.001 measured

pct q20 bases 84.2 measured

pct q30 bases 78.5 measured

gc content pct 52.1 measured

mean read length 150 measured

mean base quality 31.3 measured

adapter content pct 6.71 measured

duplication rate pct 42.05 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 56/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.5 measured ×1 43%

mean base quality 31.3 measured ×0.6 55%

adapter content pct 6.71 measured ×0.4 74%

duplication rate pct 42.05 measured ×0.4 73%

QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0