SRR13258427

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

45/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq with poor base quality and high duplication unsuitable for demanding analyses. Grade F is driven by Q30 bases at 76.3% (scored 32/100) and duplication 56.6% (scored 41/100); mean quality 30.8 is also substandard. The 150 bp reads and elevated adapter content (5.25%) suggest processing issues. Not recommended for reuse without extensive quality filtering.

Data type / assay

bulk-RNA-seq

Organism

Hormaphis cornu

Instrument

NextSeq 550

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 5190832800 reported

total reads 17302776 reported

n content pct 0.001 measured

pct q20 bases 82.2 measured

pct q30 bases 76.3 measured

gc content pct 55 measured

mean read length 150 measured

mean base quality 30.8 measured

adapter content pct 5.25 measured

duplication rate pct 56.6 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 45/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 76.3 measured ×1 32%

mean base quality 30.8 measured ×0.6 47%

adapter content pct 5.25 measured ×0.4 82%

duplication rate pct 56.6 measured ×0.4 41%

QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0