Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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SRR13258428

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

42/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

NextSeq 550 bulk RNA-Seq of Hormaphis cornu aphid with elevated GC content (59.6%) and moderate quality (82.3% Q20) across 14M reads. The dataset enables gene expression profiling in this symbiont-harboring insect but the lower base quality and potentially biased GC composition warrant careful quality filtering for downstream variant or isoform analysis. Useful for establishing baseline transcriptome composition despite moderate sequencing depth.

Data type / assay
bulk-RNA-seq
Organism
Hormaphis cornu
Instrument
NextSeq 550
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 4305932100 reported
total reads 14353107 reported
n content pct 0.001 measured
pct q20 bases 82.3 measured
pct q30 bases 76.5 measured
gc content pct 59.6 measured
mean read length 150 measured
mean base quality 30.8 measured
adapter content pct 4.89 measured
duplication rate pct 66.29 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 42/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 76.5 measured ×1 33%
mean base quality 30.8 measured ×0.6 47%
adapter content pct 4.89 measured ×0.4 84%
duplication rate pct 66.29 measured ×0.4 19%
QC cost 21 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0