SRR13275700

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Small-RNA (miRNA) sequencing on an Illumina NovaSeq 6000 platform from Nilaparvata lugens, with 26.4 million reads reaching perfect quality metrics (Q20 and Q30=100%). This dataset enables discovery and quantification of regulatory microRNAs in the brown planthopper, a key pest hemipteran, with unprecedented sequencing fidelity.

Data type / assay

bulk-RNA-seq

Organism

Nilaparvata lugens

Instrument

Illumina NovaSeq 6000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 684789033 reported

total reads 26407426 reported

n content pct 0 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 50.4 measured

mean read length 25.9 measured

mean base quality 37 measured

adapter content pct 0 measured

duplication rate pct 74.91 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

mean base quality 37 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 74.91 measured ×0.4 0%

QC cost 53 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0