Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

SRR1427160

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

This is a small-RNA (miRNA-Seq) library from mouse, sequenced on an Illumina HiSeq 2000, and overall it earns a solid B (83/100) as a usable but caveated dataset. Base quality is the main strength: Q30 of 95.5%, mean base quality of 36.4, and essentially zero adapter contamination mean the reads themselves are clean and confidently called, which is reassuring for accurate miRNA mapping. The one metric dragging the grade down is the 96% duplication rate, which scored 0/100—though for a miRNA-Seq library this is partly expected, since the small, finite repertoire of mature miRNAs and low input naturally produce many identical reads rather than indicating a failed library, so treat it as a complexity caveat rather than a disqualifier. Note that evidence_strength is 1, meaning the quality metrics here were genuinely measured rather than extrapolated, so this reading is well-grounded and not provisional, though you should still confirm the library complexity supports your specific analysis (e.g., quantifying low-abundance miRNAs) before reuse.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 219340239 reported
total reads 4300789 reported
n content pct 0.004 measured
pct q20 bases 96.2 measured
pct q30 bases 95.5 measured
gc content pct 52.7 measured
mean read length 51 measured
mean base quality 36.4 measured
adapter content pct 0 measured
duplication rate pct 96.02 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.5 measured ×1 100%
mean base quality 36.4 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 96.02 measured ×0.4 0%
QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 89