SRR1571720

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

78/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Apis mellifera bulk RNA-seq with grade C quality (78/100). Adapter contamination (14.34%) and high duplication (57.26%) jointly require preprocessing; despite strong Q30 (94.9%), both metrics will skew transcript abundance estimates. Recommend adapter trimming and duplicate collapsing before quantification.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 8292891097 reported

total reads 30081701 reported

n content pct 0.273 measured

pct q20 bases 97.5 measured

pct q30 bases 94.9 measured

gc content pct 44.9 measured

mean read length 151 measured

mean base quality 35.9 measured

adapter content pct 14.34 measured

duplication rate pct 57.26 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 78/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.9 measured ×1 100%

mean base quality 35.9 measured ×0.6 100%

adapter content pct 14.34 measured ×0.4 31%

duplication rate pct 57.26 measured ×0.4 39%

QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0