SRR1576991

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

80/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq with acceptable base quality severely compromised by exceptional duplication. Grade B reflects borderline Q30 bases (86.5%) and duplication at 64.1% (the lowest-scoring metric at 24/100), indicating PCR over-amplification or saturation of abundant transcripts. Reuse requires statistical deduplication or filters for highly expressed genes.

Data type / assay

bulk-RNA-seq

Organism

Manduca sexta

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 2929347400 reported

total reads 14646737 reported

n content pct 0.014 measured

pct q20 bases 91.7 measured

pct q30 bases 86.5 measured

gc content pct 51 measured

mean read length 100 measured

mean base quality 34.1 measured

adapter content pct 1 measured

duplication rate pct 64.1 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 80/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 86.5 measured ×1 83%

mean base quality 34.1 measured ×0.6 100%

adapter content pct 1 measured ×0.4 100%

duplication rate pct 64.1 measured ×0.4 24%

QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0