SRR1582755

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

90/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from mason bee; QC grade A marks this suitable for reuse with minor provisos. Q30 base percentage (85.8%) is the only meaningful constraint, slightly below premium but still acceptable; complemented by low duplication (8.62%) and minimal adapter contamination, supporting robust differential expression analysis.

Data type / assay

bulk-RNA-seq

Organism

Megachile rotundata

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 6459278200 reported

total reads 32296391 reported

n content pct 0.011 measured

pct q20 bases 91 measured

pct q30 bases 85.8 measured

gc content pct 39.9 measured

mean read length 100 measured

mean base quality 33.8 measured

adapter content pct 2.76 measured

duplication rate pct 8.62 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 90/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.8 measured ×1 79%

mean base quality 33.8 measured ×0.6 97%

adapter content pct 2.76 measured ×0.4 96%

duplication rate pct 8.62 measured ×0.4 100%

QC cost 31 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0