SRR1582790

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

82/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Megachile rotundata bulk RNA-seq with grade B quality (82/100). Moderate Q30 (83.7%) introduces base-call uncertainty as the primary constraint, though excellent duplication control (12.14%) and acceptable adapter (3.01%) mitigate secondary risk. Suitable for most applications with slight confidence reduction for low-frequency variants.

Data type / assay

bulk-RNA-seq

Organism

Megachile rotundata

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3799707800 reported

total reads 18998539 reported

n content pct 0.011 measured

pct q20 bases 89 measured

pct q30 bases 83.7 measured

gc content pct 40.6 measured

mean read length 100 measured

mean base quality 33 measured

adapter content pct 3.01 measured

duplication rate pct 12.14 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 82/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.7 measured ×1 69%

mean base quality 33 measured ×0.6 83%

adapter content pct 3.01 measured ×0.4 94%

duplication rate pct 12.14 measured ×0.4 100%

QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0