SRR1583606

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

91/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq with excellent base quality severely compromised by extreme duplication. Grade A reflects strong Q30 bases (94.2%) and mean quality (36.2), yet duplication at 50.86% (the limiting metric) is among the highest in this cohort and likely indicates PCR artifacts or biological saturation. Reuse requires careful deduplication handling in pipelines.

Data type / assay

bulk-RNA-seq

Organism

Aedes aegypti

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 7995750872 reported

total reads 37715806 reported

n content pct 0.012 measured

pct q20 bases 98.7 measured

pct q30 bases 94.2 measured

gc content pct 46.2 measured

mean read length 106 measured

mean base quality 36.2 measured

adapter content pct 3.18 measured

duplication rate pct 50.86 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 91/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.2 measured ×1 100%

mean base quality 36.2 measured ×0.6 100%

adapter content pct 3.18 measured ×0.4 93%

duplication rate pct 50.86 measured ×0.4 54%

QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0