SRR1596454

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

94/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq with good quality but measurable preparation artifacts. Grade A reflects solid base metrics (Q30=91.8%, mean=35.5) but duplication (41.29%) and adapter content (3.57%) indicate some library-prep bias. Suitable for reuse with deduplication; monitor for false-positive variants or fusions.

Data type / assay

bulk-RNA-seq

Organism

Anopheles gambiae

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1169146710 reported

total reads 5787855 reported

n content pct 0.01 measured

pct q20 bases 95.5 measured

pct q30 bases 91.8 measured

gc content pct 45.1 measured

mean read length 101 measured

mean base quality 35.5 measured

adapter content pct 3.57 measured

duplication rate pct 41.29 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 94/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.8 measured ×1 100%

mean base quality 35.5 measured ×0.6 100%

adapter content pct 3.57 measured ×0.4 91%

duplication rate pct 41.29 measured ×0.4 75%

QC cost 39 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0