SRR1596489

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

75/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina Genome Analyzer II bulk RNA-Seq of Anopheles gambiae generated 59M reads (11.8 Gb) with moderate quality (85% Q30, elevated N-content at 0.019%), representing earlier-generation sequencing. Despite lower per-base accuracy, the massive read count provides deep coverage for comprehensive transcriptome characterization in malaria vector biology.

Data type / assay

bulk-RNA-seq

Organism

Anopheles gambiae

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 11838656400 reported

total reads 59193282 reported

n content pct 0.019 measured

pct q20 bases 91.6 measured

pct q30 bases 85 measured

gc content pct 52.3 measured

mean read length 101 measured

mean base quality 33.2 measured

adapter content pct 13.79 measured

duplication rate pct 16.07 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 75/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85 measured ×1 75%

mean base quality 33.2 measured ×0.6 87%

adapter content pct 13.79 measured ×0.4 35%

duplication rate pct 16.07 measured ×0.4 100%

QC cost 7 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0