SRR1631064

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human skin metagenome WGS with grade C quality (79/100). Adapter contamination at 28.17% is the critical failure point, indicating incomplete library cleanup that inflates sequence complexity and risks artifactual diversity overestimation in microbial composition. The excellent duplication rate (1.11%) and Q30 (91%) suggest sound underlying sequencing quality after preprocessing.

Data type / assay

WGS

Organism

human skin metagenome

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 5530395996 reported

total reads 27378198 reported

n content pct 0.576 measured

pct q20 bases 97 measured

pct q30 bases 91 measured

gc content pct 53.9 measured

mean read length 101 measured

mean base quality 35.3 measured

adapter content pct 28.17 measured

duplication rate pct 1.11 measured

How this grade was computed

Weighted mean of 3 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91 measured ×1 100%

duplication rate pct 1.11 measured ×0.5 100%

adapter content pct 28.17 measured ×0.4 0%

QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0