Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRR1647953

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

88/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

This is a small-RNA (miRNA-Seq) library from mouse profiled on an Illumina HiSeq 2500, and it earns a solid B (88/100) on a fully measured QC pass (evidence_strength=1), so the reading is firm rather than provisional. Base quality is the dataset's strength — 95.4% of bases at Q30, a mean base quality of 37.8, and effectively zero adapter contamination (0%) despite the short 51 bp reads, meaning the per-base calls are trustworthy and reads are clean for mapping. The single factor dragging the grade down is a 62.39% duplication rate, which scored only 28/100; while high duplication is partly expected and biologically plausible in miRNA-Seq because a small repertoire of short mature miRNAs is sequenced very deeply, it still caps library complexity and can bias quantification, so you should rely on UMI-aware or duplication-tolerant counting if reusing these data. Overall the data are reusable for miRNA expression analysis, but treat duplication as the key caveat rather than a disqualifier.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 255735369 reported
total reads 5014419 reported
n content pct 0.019 measured
pct q20 bases 97.4 measured
pct q30 bases 95.4 measured
gc content pct 48.8 measured
mean read length 51 measured
mean base quality 37.8 measured
adapter content pct 0 measured
duplication rate pct 62.39 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 88/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.4 measured ×1 100%
mean base quality 37.8 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 62.39 measured ×0.4 28%
QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 89