SRR166828

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

71/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Genome Analyzer II RNA-seq of *Drosophila pseudoobscura* produces 24.4M reads and 4.92 Gb bases with 92.6% Q20, allowing comparative transcript mapping across Drosophila species for studying regulatory evolution and developmental programs. Search: *Drosophila pseudoobscura* RNA-seq, Drosophila transcriptome, evolutionary genomics.

Data type / assay

bulk-RNA-seq

Organism

Drosophila pseudoobscura

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 4919804738 reported

total reads 24355469 reported

n content pct 0.058 measured

pct q20 bases 92.6 measured

pct q30 bases 85.5 measured

gc content pct 50.7 measured

mean read length 101 measured

mean base quality 34.3 measured

adapter content pct 45 measured

duplication rate pct 38.72 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 71/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.5 measured ×1 78%

mean base quality 34.3 measured ×0.6 100%

adapter content pct 45 measured ×0.4 0%

duplication rate pct 38.72 measured ×0.4 81%

QC cost 31 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0