SRR1783723

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

37/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

454 long-read RNA-seq from Bactrocera dorsalis with grade-F due to platform-intrinsic quality limits: Q30 of 60.7% and mean base quality 30 mean ~40% of bases are <Q30. Combined with 43.3% duplication and 454's error profile, basecall reliability is compromised. Not recommended for reuse without custom error modeling and careful validation.

Data type / assay

bulk-RNA-seq

Organism

Bactrocera dorsalis

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 200439678 reported

total reads 325382 reported

n content pct 0.05 measured

pct q20 bases 84.8 measured

pct q30 bases 60.7 measured

gc content pct 38.2 measured

mean read length 293.2 measured

mean base quality 30 measured

adapter content pct 0 measured

duplication rate pct 43.33 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 37/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 60.7 measured ×1 0%

mean base quality 30 measured ×0.6 33%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 43.33 measured ×0.4 70%

QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0