Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

SRR1820680

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

This is a human miRNA-Seq library (small-RNA Illumina HiSeq 2000 run), and on QC grounds it lands as a borderline-fail (D, 67/100) that is genuinely usable but with an important caveat. The base chemistry is excellent — pct_q30 of 97.2% and mean base quality of 38.3 show the sequencer called bases very confidently, so raw signal quality is not the problem. What drags the grade down are adapter_content at 97.45% and duplication_rate at 91.74%, both of which scored zero; for a 50 bp small-RNA library this is actually expected behaviour (mature miRNAs are ~22 nt, so reads run straight into adapter, and high duplication reflects a few highly abundant miRNAs rather than PCR artifact), but it means you cannot reuse these reads without aggressive adapter trimming and miRNA-aware deduplication, and a generic RNA-seq pipeline would choke. Note also that the evidence_strength is low (1) with total_reads, total_bases and checksum only reported rather than measured, so treat throughput and depth as provisional pending a full measured pass before you rely on this for quantitative miRNA expression.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 315553000 reported
total reads 6311060 reported
n content pct 0.008 measured
pct q20 bases 98.9 measured
pct q30 bases 97.2 measured
gc content pct 46.7 measured
mean read length 50 measured
mean base quality 38.3 measured
adapter content pct 97.45 measured
duplication rate pct 91.74 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.2 measured ×1 100%
mean base quality 38.3 measured ×0.6 100%
adapter content pct 97.45 measured ×0.4 0%
duplication rate pct 91.74 measured ×0.4 0%
QC cost 12 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 89