SRR1951513

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

93/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq short-read (fly). Grade A despite measured duplication of 48.24% (component score 60/100). Excellent Q30 (97.9%, measured), zero adapters (measured), and 37.9 mean quality (measured) indicate duplication is primarily PCR-driven at library stage; suitable for expression analysis with duplicate-aware tools (Sambamba, featureCounts).

Data type / assay

bulk-RNA-seq

Organism

Calliphora vicina

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 461420736 reported

total reads 4977231 reported

n content pct 0.001 measured

pct q20 bases 99.6 measured

pct q30 bases 97.9 measured

gc content pct 25.7 measured

mean read length 91.2 measured

mean base quality 37.9 measured

adapter content pct 0 measured

duplication rate pct 48.24 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 93/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.9 measured ×1 100%

mean base quality 37.9 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 48.24 measured ×0.4 60%

QC cost 6 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0