SRR1951515

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

93/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

High-quality bulk RNA-seq from Calliphora vicina (A grade, 93/100) with excellent Q30 (97.8%) and base quality, but moderate read duplication (48.3%, scored 59/100) is the primary limitation. This duplication level appears consistent within the Calliphora samples and may reflect genuine transcript complexity; reuse is supported with awareness of duplication patterns.

Data type / assay

bulk-RNA-seq

Organism

Calliphora vicina

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 446202995 reported

total reads 4836716 reported

n content pct 0 measured

pct q20 bases 99.6 measured

pct q30 bases 97.8 measured

gc content pct 25.3 measured

mean read length 91.1 measured

mean base quality 37.9 measured

adapter content pct 0 measured

duplication rate pct 48.33 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 93/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.8 measured ×1 100%

mean base quality 37.9 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 48.33 measured ×0.4 59%

QC cost 36 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0