SRR2006622

ENA first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

71/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq with moderately degraded Q30 quality (80.1%, scoring 50/100) and borderline mean base quality (Q32.1), limiting downstream isoform-level analysis. Duplication is clean (8.64%); all metrics are measured with full evidence. Reuse is restricted to robust differential-expression analysis or gene-level abundance studies, not transcript discovery.

Data type / assay

bulk-RNA-seq

Organism

Carya cathayensis

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 4674938116 reported

total reads 23143258 reported

n content pct 0.038 measured

pct q20 bases 89 measured

pct q30 bases 80.1 measured

gc content pct 53.4 measured

mean read length 101 measured

mean base quality 32.1 measured

adapter content pct 0.49 measured

duplication rate pct 8.64 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 71/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 80.1 measured ×1 51%

mean base quality 32.1 measured ×0.6 68%

adapter content pct 0.49 measured ×0.4 100%

duplication rate pct 8.64 measured ×0.4 100%

QC cost 48 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0