Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR2029826

ENA first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

71/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Sistrurus tergeminus (massasauga rattlesnake) RNA-seq from HiSeq 2000 with exceptional depth (22.1B bases, 110.9M reads) and 82.7% Q30 — essentially a small vertebrate genome equivalent. This ultra-deep transcriptome is excellent for comprehensive gene annotation, splicing characterization, and reptile comparative genomics; search for snake transcriptomics or non-mammalian vertebrate expression.

Data type / assay
bulk-RNA-seq
Organism
Sistrurus tergeminus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 22199450600 reported
total reads 110997253 reported
n content pct 0.002 measured
pct q20 bases 87.5 measured
pct q30 bases 82.7 measured
gc content pct 45.4 measured
mean read length 100 measured
mean base quality 32.8 measured
adapter content pct 4.45 measured
duplication rate pct 46.71 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 71/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.7 measured ×1 64%
mean base quality 32.8 measured ×0.6 80%
adapter content pct 4.45 measured ×0.4 86%
duplication rate pct 46.71 measured ×0.4 63%
QC cost 50 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0