SRR2050391

ENA first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

31/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

454 GS FLX whole-genome sequencing of Escherichia coli producing 55 million bases from 218k longer reads with mixed quality (93.2% Q20, 23.3% Q30). This long-read WGS dataset may be valuable for resolving repetitive regions and structural variants but requires careful quality trimming and validation, particularly given the low Q30 percentage typical of this platform.

Data type / assay

WGS

Organism

Escherichia coli

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 55709484 reported

total reads 218501 reported

mean coverage 12.1 extrapolated

n content pct 0.008 measured

pct q20 bases 93.2 measured

pct q30 bases 23.3 measured

gc content pct 50.8 measured

mean read length 251.8 measured

mean base quality 27.3 measured

adapter content pct 0 measured

duplication rate pct 18.19 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 31/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

mean coverage 12.1 extrapolated ×1.2 19%

pct q30 bases 23.3 measured ×1 0%

duplication rate pct 18.19 measured ×0.5 68%

adapter content pct 0 measured ×0.4 100%

QC cost 22 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0