Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR2138420

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

99/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Drosophila pseudoobscura pseudoobscura using HiSeq 1000, generating 89 million reads at exceptional quality (93.2% Q30). This deep transcriptomic dataset enables fine-resolution isoform quantification, de novo transcriptome assembly, and cross-species expression comparisons, particularly powerful for studying gene expression evolution in non-reference Drosophila.

Data type / assay
bulk-RNA-seq
Organism
Drosophila pseudoobscura pseudoobscura
Instrument
Illumina HiSeq 1000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 18043161766 reported
total reads 89322583 reported
n content pct 0.017 measured
pct q20 bases 96.3 measured
pct q30 bases 93.2 measured
gc content pct 50.6 measured
mean read length 101 measured
mean base quality 35.8 measured
adapter content pct 0.47 measured
duplication rate pct 31.58 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 99/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.2 measured ×1 100%
mean base quality 35.8 measured ×0.6 100%
adapter content pct 0.47 measured ×0.4 100%
duplication rate pct 31.58 measured ×0.4 97%
QC cost 44 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0