SRR2138436

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Drosophila pseudoobscura pseudoobscura RNA-seq from HiSeq 1000 with 4.8B bases and 92.4% Q30, supporting solid isoform and differential expression studies in this model species for speciation genomics. The data are well-suited for comparative transcriptomics with D. melanogaster.

Data type / assay

bulk-RNA-seq

Organism

Drosophila pseudoobscura pseudoobscura

Instrument

Illumina HiSeq 1000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 4834122600 reported

total reads 23931300 reported

n content pct 0.015 measured

pct q20 bases 96 measured

pct q30 bases 92.4 measured

gc content pct 50.9 measured

mean read length 101 measured

mean base quality 35.5 measured

adapter content pct 0.58 measured

duplication rate pct 11.49 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.4 measured ×1 100%

mean base quality 35.5 measured ×0.6 100%

adapter content pct 0.58 measured ×0.4 100%

duplication rate pct 11.49 measured ×0.4 100%

QC cost 31 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0