SRR2820618

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

66/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

WGS from Klebsiella on HiSeq 2000. D-grade primarily due to borderline base quality (77.1% Q30), falling below thresholds for confident variant calling. While adapter and duplication metrics are controlled, the quality deficit limits genomic accuracy. Conditional reuse only with aggressive quality filtering.

Data type / assay

WGS

Organism

Klebsiella aerogenes

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 790183196 reported

total reads 3911798 reported

n content pct 0.001 measured

pct q20 bases 90.6 measured

pct q30 bases 77.1 measured

gc content pct 53.6 measured

mean read length 101 measured

mean base quality 31.6 measured

adapter content pct 0.09 measured

duplication rate pct 5.99 measured

How this grade was computed

Weighted mean of 3 scored metric(s) → 66/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 77.1 measured ×1 36%

duplication rate pct 5.99 measured ×0.5 100%

adapter content pct 0.09 measured ×0.4 100%

QC cost 38 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0