SRR2895251

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Osmia bicornis mason bee RNA-seq via HiSeq 2000 (27.7M reads, 5.59B bases, 97.9% Q20, 94.3% Q30). The combination of high sequencing depth, excellent quality, and 40.2% GC composition provides comprehensive transcriptome coverage for studying solitary bee physiology and comparing gene regulatory networks with social Apis species.

Data type / assay

bulk-RNA-seq

Organism

Osmia bicornis

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 5590298086 reported

total reads 27674743 reported

n content pct 0.284 measured

pct q20 bases 97.9 measured

pct q30 bases 94.3 measured

gc content pct 40.2 measured

mean read length 101 measured

mean base quality 36.5 measured

adapter content pct 0.16 measured

duplication rate pct 17.49 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.3 measured ×1 100%

mean base quality 36.5 measured ×0.6 100%

adapter content pct 0.16 measured ×0.4 100%

duplication rate pct 17.49 measured ×0.4 100%

QC cost 31 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0