SRR305922

ENA first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

47/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Long-read PacBio WGS from E. coli; F-grade unsuitable for assembly. Q30 base accuracy (23.2%) and mean base quality (21.1) are critically low for PacBio standards, precluding reliable variant calling or assembly.

Data type / assay

WGS

Organism

Escherichia coli O104:H4 str. C227-11

Instrument

PacBio RS

Platform

PACBIO_SMRT

Read type

long-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 155125847 reported

total reads 81741 reported

n content pct 0 measured

pct q20 bases 48.9 measured

pct q30 bases 23.2 measured

gc content pct 51.3 measured

mean read length 1325.6 measured

mean base quality 21.1 measured

adapter content pct 0 measured

duplication rate pct 0 measured

How this grade was computed

Weighted mean of 3 scored metric(s) → 47/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 23.2 measured ×1 0%

duplication rate pct 0 measured ×0.5 100%

adapter content pct 0 measured ×0.4 100%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0