Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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SRR305923

ENA first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

47/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Long-read WGS from PacBio (E. coli, F grade, 47/100). Q30 bases (25.8%) scores zero, and mean base quality (22) indicates weak consensus—even for raw long reads these measured metrics signal sequencing or instrument issues that undermine assembly quality. The zero duplication and adapters are expected for long reads but do not offset the poor quality. Reuse requires validation.

Data type / assay
WGS
Organism
Escherichia coli O104:H4 str. C227-11
Instrument
PacBio RS
Platform
PACBIO_SMRT
Read type
long-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 148734095 reported
total reads 81741 reported
n content pct 0 measured
pct q20 bases 49.6 measured
pct q30 bases 25.8 measured
gc content pct 51.6 measured
mean read length 1312.9 measured
mean base quality 22 measured
adapter content pct 0 measured
duplication rate pct 0 measured
How this grade was computed
Weighted mean of 3 scored metric(s) → 47/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 25.8 measured ×1 0%
duplication rate pct 0 measured ×0.5 100%
adapter content pct 0 measured ×0.4 100%
QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0