SRR3123341

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

38/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq with severely degraded Q30 quality (59.7%, scoring 0/100) and marginal mean base quality (Q29.2, scoring 20/100), exceeding even its sister sample SRR3123357's quality loss; elevated duplication (29.4%) compounds the issue, suggesting run-end or instrument failure. Dataset is unsuitable for reuse.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3329745103 reported

total reads 16485479 reported

n content pct 0.008 measured

pct q20 bases 89.8 measured

pct q30 bases 59.7 measured

gc content pct 41.7 measured

mean read length 101 measured

mean base quality 29.2 measured

adapter content pct 0 measured

duplication rate pct 29.4 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 38/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 59.7 measured ×1 0%

mean base quality 29.2 measured ×0.6 20%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 29.4 measured ×0.4 100%

QC cost 22 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0