SRR3123364

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

38/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

HiSeq 2500 RNA-seq from Apis mellifera (18.2M reads, 3.7 billion bases, 38.9% GC, 59.8% ≥Q30) with surprisingly low Q30 despite high Q20 indicates marginal read accuracy. The dataset requires conservative quality filtering for reliable analysis.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3681656879 reported

total reads 18227490 reported

n content pct 0.005 measured

pct q20 bases 89.6 measured

pct q30 bases 59.8 measured

gc content pct 38.9 measured

mean read length 101 measured

mean base quality 29.2 measured

adapter content pct 0 measured

duplication rate pct 13.69 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 38/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 59.8 measured ×1 0%

mean base quality 29.2 measured ×0.6 20%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 13.69 measured ×0.4 100%

QC cost 32 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0