SRR3123372

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

38/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina HiSeq 2500 RNA-seq of Apis mellifera honey bee yielding ~3.38 billion bases and ~16.7 million reads; note the moderate Q30 (59.7%) typical of this run, adequate for primary transcriptome assembly but limiting variant confidence.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3375828846 reported

total reads 16713367 reported

n content pct 0.004 measured

pct q20 bases 89.7 measured

pct q30 bases 59.7 measured

gc content pct 40 measured

mean read length 101 measured

mean base quality 29.2 measured

adapter content pct 0 measured

duplication rate pct 15.92 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 38/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 59.7 measured ×1 0%

mean base quality 29.2 measured ×0.6 20%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 15.92 measured ×0.4 100%

QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0