Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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SRR3144559

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

49/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Short-read RNA-seq on the HiSeq 4000 platform captures the Apis mellifera transcriptome at 15 million reads with moderate base quality (84% Q20, 69% Q30), adequate for standard gene expression profiling across colonies or developmental stages. The honeybee dataset supports comparative transcriptomics in this social insect model. Q30 below optimal thresholds suggests reduced confidence for low-frequency variant calls; filtering or trimming is recommended for sensitive work.

Data type / assay
bulk-RNA-seq
Organism
Apis mellifera
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 3889864944 reported
total reads 15435972 reported
n content pct 0.009 measured
pct q20 bases 84 measured
pct q30 bases 69 measured
gc content pct 44.5 measured
mean read length 126 measured
mean base quality 31.7 measured
adapter content pct 0 measured
duplication rate pct 22.63 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 49/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 69 measured ×1 0%
mean base quality 31.7 measured ×0.6 62%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 22.63 measured ×0.4 100%
QC cost 1 min compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0