SRR3180029

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

80/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

HiSeq 2500 RNA-seq from the whitefly Bemisia tabaci yields ~300 bp average reads (high for short-read RNA-seq) with decent quality (94% Q20, 90.8% Q30), enabling transcript assembly and expression studies in this economically important crop pest.

Data type / assay

bulk-RNA-seq

Organism

Bemisia tabaci

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3165100430 reported

total reads 10480465 reported

n content pct 0.046 measured

pct q20 bases 94 measured

pct q30 bases 90.8 measured

gc content pct 40.7 measured

mean read length 151 measured

mean base quality 35 measured

adapter content pct 26.13 measured

duplication rate pct 39.1 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 80/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.8 measured ×1 100%

mean base quality 35 measured ×0.6 100%

adapter content pct 26.13 measured ×0.4 0%

duplication rate pct 39.1 measured ×0.4 80%

QC cost 41 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0