SRR3222649

ENA first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

86/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human WGS; B-grade overall but reading is provisional—mean coverage (43.9×) is extrapolated, not measured. Critical issue: adapter contamination (67.3%, scored 0/100) indicates likely technical contamination or demultiplexing error requiring upstream investigation before reuse.

Data type / assay

WGS

Organism

Homo sapiens

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 135986969100 reported

total reads 453289897 reported

mean coverage 43.9 extrapolated

n content pct 0.023 measured

pct q20 bases 94.5 measured

pct q30 bases 91.2 measured

gc content pct 53.7 measured

mean read length 150 measured

mean base quality 38.7 measured

adapter content pct 67.27 measured

duplication rate pct 9.42 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 86/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

mean coverage 43.9 extrapolated ×1.2 100%

pct q30 bases 91.2 measured ×1 100%

duplication rate pct 9.42 measured ×0.5 96%

adapter content pct 67.27 measured ×0.4 0%

QC cost 36 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0