SRR3289627

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

87/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

HiSeq 2000 RNA-seq from Tribolium castaneum with 184 bp reads, solid quality (94.1% Q20, 84.7% Q30), and 18.7 million reads, enabling reliable gene expression profiling in this arthropod developmental and genomic model.

Data type / assay

bulk-RNA-seq

Organism

Tribolium castaneum

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3436689100 reported

total reads 18676250 reported

n content pct 0.003 measured

pct q20 bases 94.1 measured

pct q30 bases 84.7 measured

gc content pct 44 measured

mean read length 100 measured

mean base quality 33.9 measured

adapter content pct 0 measured

duplication rate pct 33.97 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 87/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84.7 measured ×1 74%

mean base quality 33.9 measured ×0.6 98%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 33.97 measured ×0.4 91%

QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0