SRR3323584

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

82/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq from beetle (Dendroctonus ponderosae, B grade, 82/100). Q30 bases (83.5%) scores only 68/100 (measured), limiting base quality for allele-specific or structural analysis. The elevated duplication rate (33.59%) reduces effective coverage due to transcript abundance. Suitable for differential expression but edge cases require validation.

Data type / assay

bulk-RNA-seq

Organism

Dendroctonus ponderosae

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 4937999675 reported

total reads 32869809 reported

n content pct 0.003 measured

pct q20 bases 91.5 measured

pct q30 bases 83.5 measured

gc content pct 45.3 measured

mean read length 75.1 measured

mean base quality 33.2 measured

adapter content pct 0.02 measured

duplication rate pct 33.59 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 82/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.5 measured ×1 68%

mean base quality 33.2 measured ×0.6 87%

adapter content pct 0.02 measured ×0.4 100%

duplication rate pct 33.59 measured ×0.4 92%

QC cost 20 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0