Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR3342470

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of mountain pine beetle *Dendroctonus ponderosae* on Illumina NextSeq 500 with 20.7M reads (3.11 Gb), good base quality (83.2% Q30), and 44.3% GC, providing complementary transcriptome data for this economically important forest pest. NextSeq platform offers cost-effective sequencing for comparative insect transcriptomics. Search: beetle gene expression, pest biology, forest genomics.

Data type / assay
bulk-RNA-seq
Organism
Dendroctonus ponderosae
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 3109778821 reported
total reads 20708211 reported
n content pct 0.003 measured
pct q20 bases 91.3 measured
pct q30 bases 83.2 measured
gc content pct 44.3 measured
mean read length 75.1 measured
mean base quality 33.2 measured
adapter content pct 0.02 measured
duplication rate pct 29.88 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.2 measured ×1 66%
mean base quality 33.2 measured ×0.6 87%
adapter content pct 0.02 measured ×0.4 100%
duplication rate pct 29.88 measured ×0.4 100%
QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0