SRR3355252

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

96/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

High-quality bulk RNA-seq with excellent measured base quality (Q37.2, 95.1% Q30) and negligible adapter content (0.16%), suitable for isoform-level analysis. The moderately elevated duplication (39.47%, scoring 79/100) is the limiting factor, likely reflecting biological duplicate transcripts rather than technical artifact; measured high evidence (1.0) supports confident reuse.

Data type / assay

bulk-RNA-seq

Organism

Drosophila grimshawi

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1281317820 reported

total reads 16859445 reported

n content pct 0.012 measured

pct q20 bases 98.7 measured

pct q30 bases 95.1 measured

gc content pct 49.9 measured

mean read length 76 measured

mean base quality 37.2 measured

adapter content pct 0.16 measured

duplication rate pct 39.47 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 96/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.1 measured ×1 100%

mean base quality 37.2 measured ×0.6 100%

adapter content pct 0.16 measured ×0.4 100%

duplication rate pct 39.47 measured ×0.4 79%

QC cost 1 min compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0