Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRR3402132

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

76/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

Bulk small-RNA (miRNA-Seq) data from mouse on an Illumina HiSeq 2500, scored 76/100 (grade C). The grade is dragged down almost entirely by an 86.47% duplication rate, which scores 0 and is the dominant negative driver — though for miRNA-Seq this is partly expected, since the small, finite repertoire of mature miRNAs and short ~51 bp reads inflate apparent PCR/optical duplication, so it should not be read as harshly as it would for a standard mRNA library. Working in the data's favor are excellent base quality (mean Q34.9, ~86.6% of bases ≥Q30) and zero adapter contamination, both scoring full marks and indicating clean, reliably basecalled reads suitable for accurate miRNA alignment and quantification. Note that evidence_strength is 1, meaning all scored metrics were directly measured rather than extrapolated, so this reading is firm rather than provisional — overall the dataset is reusable for miRNA expression analysis provided you treat the high duplication as assay-intrinsic and apply UMI- or count-based normalization rather than naive dedup.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 753314166 reported
total reads 14770866 reported
n content pct 0.278 measured
pct q20 bases 90.3 measured
pct q30 bases 86.6 measured
gc content pct 53.4 measured
mean read length 51 measured
mean base quality 34.9 measured
adapter content pct 0 measured
duplication rate pct 86.47 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 76/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 86.6 measured ×1 83%
mean base quality 34.9 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 86.47 measured ×0.4 0%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 89