SRR3425165

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

87/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Tsetse-fly bulk RNA-seq; B-grade quality with strong base metrics (Q30=94.6%). Duplication rate (64.1%, scored 24/100) is elevated, suggesting possible PCR bias; suitable for analysis with appropriate downstream corrections.

Data type / assay

bulk-RNA-seq

Organism

Glossina morsitans morsitans

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1706411964 reported

total reads 22452789 reported

n content pct 0.003 measured

pct q20 bases 98 measured

pct q30 bases 94.6 measured

gc content pct 36.5 measured

mean read length 76 measured

mean base quality 37.2 measured

adapter content pct 0.02 measured

duplication rate pct 64.06 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 87/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.6 measured ×1 100%

mean base quality 37.2 measured ×0.6 100%

adapter content pct 0.02 measured ×0.4 100%

duplication rate pct 64.06 measured ×0.4 24%

QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0