SRR3439429

ENA first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

82/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human bulk RNA-seq on Illumina HiSeq 4000 with 41.4M reads (12.3 Gb), moderate base quality (83% Q30), sufficient for transcript discovery and gene-level expression profiling. Employ quality filtering for isoform-level or splice-junction analysis. Applications: human transcriptomics, gene expression, functional genomics.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina HiSeq 4000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 12251801393 reported

total reads 41358284 reported

n content pct 0.006 measured

pct q20 bases 90.3 measured

pct q30 bases 83 measured

gc content pct 43.4 measured

mean read length 148.2 measured

mean base quality 33.1 measured

adapter content pct 0.07 measured

duplication rate pct 16.01 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 82/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83 measured ×1 65%

mean base quality 33.1 measured ×0.6 85%

adapter content pct 0.07 measured ×0.4 100%

duplication rate pct 16.01 measured ×0.4 100%

QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0