SRR3439475

ENA first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human bulk RNA-seq with borderline Q30 quality (83.2%, scoring 66/100) and Q33 mean base quality, limiting confidence in lowly-expressed isoform calls and rare variant detection. Duplication is acceptable (27.42%); all metrics are measured with full evidence. Reuse is viable for robust expression studies but not for sensitive fusion detection or splice-variant discovery.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina HiSeq 4000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 8714470391 reported

total reads 29751401 reported

n content pct 0.001 measured

pct q20 bases 90.6 measured

pct q30 bases 83.2 measured

gc content pct 44.7 measured

mean read length 146.6 measured

mean base quality 33.2 measured

adapter content pct 0.1 measured

duplication rate pct 27.42 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.2 measured ×1 66%

mean base quality 33.2 measured ×0.6 87%

adapter content pct 0.1 measured ×0.4 100%

duplication rate pct 27.42 measured ×0.4 100%

QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0