SRR3467219

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

98/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from Manduca sexta on an Illumina HiSeq 2000, yielding 32.5 million reads spanning 1.6 billion bases with high Q30 content (97.4%). With moderate N-content (0.011%) and 47.1% GC composition, this dataset enables expression profiling in lepidopteran development.

Data type / assay

bulk-RNA-seq

Organism

Manduca sexta

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1591062928 reported

total reads 32470672 reported

n content pct 0.011 measured

pct q20 bases 99 measured

pct q30 bases 97.4 measured

gc content pct 47.1 measured

mean read length 49 measured

mean base quality 38.3 measured

adapter content pct 0 measured

duplication rate pct 36.7 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 98/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.4 measured ×1 100%

mean base quality 38.3 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 36.7 measured ×0.4 85%

QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0