SRR350019

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

80/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read RNA-seq from Lucilia sericata on an Illumina Genome Analyzer II, yielding 17.7 million reads at maximum Q30 quality (100%) across 953 million bases. The elevated N-content (0.076%) reflects older sequencing, but the balanced GC composition (39.7%) and read depth support expression studies in this blowfly.

Data type / assay

bulk-RNA-seq

Organism

Lucilia sericata

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 953389980 reported

total reads 17655370 reported

n content pct 0.076 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 39.7 measured

mean read length 54 measured

mean base quality 30 measured

adapter content pct 0.15 measured

duplication rate pct 39.37 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 80/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

mean base quality 30 measured ×0.6 33%

adapter content pct 0.15 measured ×0.4 100%

duplication rate pct 39.37 measured ×0.4 79%

QC cost 38 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0